Bayesian experimental design for optimizing medium composition and biomass formation of tobacco BY-2 cell suspension cultures in stirred-tank bioreactors.

ABSTRACT:

Introduction

The plant suspension cell culture Bright Yellow-2 (BY-2) can be an economic platform for producing complex biopharmaceuticals like cytokines and antibodies at small- and medium-scale because of potentially reduced cultivation and purification costs compared to mammalian cells. This is especially relevant for rare diseases. However, the productivity is currently low in terms of biomass formation in a typical batch fermentation. A potential reason might be that the standard BY-2 cultivation medium, as it is used under laboratory shake-flask cultivation conditions, has not yet been comprehensively optimized and tested in industrial bioreactor settings, addressing all four macronutrients relevant for biomass formation (i.e., sucrose, ammonium, nitrate, and phosphate) in pa

SUBMITTER: Leyendecker L 

PROVIDER: S-EPMC12492029 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature