Transposition of insertion sequence element from KPC plasmid enhances intracellular survival of Klebsiella pneumoniae.

ABSTRACT:

Background

The convergence of plasmids encoding Klebsiella pneumoniae carbapenemase (KPC) and virulence determinants is increasingly reported in K. pneumoniae (Kpn). However, how KPC plasmids interfere with the virulence plasmid-mediated pathogen-host interactions, and the significance of this interference in the within-host adaption of hypervirulent carbapenem-resistant K. pneumoniae (Hv-CR-Kpn), remain unclear.

Methods

An Hv-CR-Kpn variant based on ST11 CR-Kpn was created to determine the impact of capsular polysaccharide (CPS) in Kpn intracellular proliferation. Whole genome sequencing was conducted to find the cause of CPS loss. The biological significance of spontaneous CPS loss in Hv-CR-Kpn was ascertained with cell lines and murine model.

Findings

The acquisiti

SUBMITTER: Xie Y 

PROVIDER: S-EPMC12492035 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature