An Online Buffer Exchange Platform for Charge Detection Mass Spectrometry Analysis of AAVs and AAV-Antibody Complexes.

ABSTRACT: Adeno-associated viruses (AAVs) are leading vectors in gene therapy that have demonstrated great potential in combating a wide range of human diseases. To enhance specificity and reduce dosing requirements, antibody-retargeted AAVs have emerged as a promising strategy to redirect vectors to novel receptors, thereby achieving improved efficacy and safety. However, effective characterization of AAVs and AAV-antibody complexes is complicated by heterogeneities that arise from variations in capsid protein assembly, genome integrity, and antibody decorations, demanding high-resolution techniques beyond traditional methods. Charge detection mass spectrometry (CDMS) is an emerging technique that effectively characterizes complex AAV samples by directly measuring ion charge. This technique is typi

SUBMITTER: Du C 

PROVIDER: S-EPMC12492384 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature