Evaluation of <i>in vitro</i> and <i>in vivo</i> release of recombinant human nerve growth factor from bioengineered human stromal lenticule.

ABSTRACT:

Introduction

Small incision lenticule extraction (SMILE)-derived lenticules have been repurposed as biocompatible scaffolds to incorporate and release therapeutic substances for ocular therapeutics. We aim to investigate the in vitro and in vivo release profiles of recombinant human nerve growth factor (rhNGF) from bioengineered human stromal lenticules prepared with microparticles incorporated with rhNGF (rhNGF-MPs) for up to 1 and 4 weeks, respectively.

Methods

Upon bioengineered lenticule implantation, slit lamp, Anterior Segment Optical Coherence Tomography, and In Vivo Confocal Microscopy were performed to assess corneal biocompatibility, central corneal thickness (CCT), corneal nerve -fiber density (CNFD), -branch density, and -fiber length. Rabbit

SUBMITTER: Lin MT 

PROVIDER: S-EPMC12492493 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature