Repression of mab_1999 impairs growth and alters cellular morphology of Mycobacterium abscessus.

ABSTRACT:

Background

Cell division is essential for bacterial survival and represents a promising target for the development of novel antibiotics, particularly in mycobacteria. The role of the division protein FtsL in Mycobacterium abscessus remains poorly understood. This study investigated the effects of MAB_1999, a predicted homolog of FtsL, on the growth and cell division of M. abscessus.

Method

To investigate the function of mab_1999, a knockdown mutant was generated via CRISPR interference (CRISPRi). The phenotypic impact of mab_1999 suppression was evaluated, with a focus on its effects on M. abscessus growth, cellular morphology, and antibiotic susceptibility.

Results

The putative homolog of FtsL in M. abscessus (MAB_1999) shares 54% amino acid sequence identity with Ft

SUBMITTER: Ilmi AFN 

PROVIDER: S-EPMC12492608 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature