Investigation of prevalence and phylogenetic classification of Microsporidia MB and insecticide target site insensitivity resistance mutations in Anopheles gambiae s.l. and Anopheles funestus mosquitoes from Busia, Kenya.

ABSTRACT:

Background

Microsporidia MB, a Plasmodium-transmission-impairing symbiont in Anopheles arabiensis, has malaria control potential. This study assessed its prevalence and phylogeny in An. gambiae s.l. and An. funestus in Busia, Kenya and investigated the influence of environmental factors on its occurrence. Additionally, the prevalence of key insecticide resistance mutations in these mosquito populations was determined.

Methods

Mosquito larvae and adults were collected from three sub-counties in Busia County, Kenya and identified based on morphological characteristics. PCR was used to determine Anopheles species distribution and Microsporidia MB prevalence following DNA extraction from the samples. Insecticide resistance target-site mutations were identified using TaqMan genot

SUBMITTER: Wandera HT 

PROVIDER: S-EPMC12492619 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature