Integrative transcriptomic analysis reveals cross-species conserved core genes and pathways in alveolar macrophages during ALI/ARDS.

ABSTRACT:

Background and purpose

acute respiratory distress syndrome (ARDS) is a severe pulmonary condition characterized by alveolar-capillary damage and refractory hypoxemia. Alveolar macrophages (AMs) play a crucial role in regulating inflammation and repair processes during acute lung injury (ALI)/ARDS. However, the transcriptional and functional changes in AMs during ALI/ARDS remain poorly understood, especially when considering species-specific differences between murine models and human pathophysiology. This study aims to elucidate these changes in AMs during ALI/ARDS by integrating in vitro and cross-species transcriptomic analyses.

Methods

We conducted RNA sequencing on LPS-stimulated MH-S cells and integrated the data with publicly available murine (GSE225406) and human (GSE

SUBMITTER: Li A 

PROVIDER: S-EPMC12492643 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature