Engineering <i>Yarrowia lipolytica</i> as a yeast cell factory for the de novo production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate).

ABSTRACT: The oleaginous yeast Yarrowia lipolytica presents a promising chassis for the production of biodegradable plastic polyhydroxyalkanoates (PHAs) due to its advantages in precursor supply capabilities and cost-effective substrate utilization. However, Y. lipolytica has primarily been engineered for the synthesis of homopolymeric PHAs, such as poly (3-hydroxybutyrate) (P3HB), which have limited material properties and application potential. In this study, the succinate dehydrogenase-deficient Y. lipolytica strains were utilized as hosts for the de novo biosynthesis of copolymer poly (3-hydroxybutyrate-co-4-hydroxybutyrate) (P34HB). By employing a strategy of subcellular partitioning, poly (4-hydroxybutyrate) (P4HB) was synthesized in the cytoplasm using 4-hydrobutyrate (4H

SUBMITTER: Liu J 

PROVIDER: S-EPMC12492649 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature