Topoisomerase II-Like variants enhance acid tolerance and low-pH l-malic acid biosynthesis in metabolic engineered <i>Aspergillus nidulans</i>.

ABSTRACT: l-malic acid is a versatile compound having extensive applications across food, pharmaceutical, medical, and chemical industries while its production from microbial fermentation offers sustainable advantages including renewable feedstocks and environmental friendliness. However, a major challenge faced by microbial l-malic acid production is acid-induced pH drop during the fermentation process, requiring excessive neutralizers (e.g., CaCO₃) that increase costs and environmental burdens greatly. In this study, we firstly made an engineered Aspergillus nidulans strain as a production chassis through metabolic engineering by overexpression of fructose-6-phosphate kinase gene (pfkA) combined with deletion of pyruvate decarboxylase gene (pdcA) so that the titer o

SUBMITTER: Pei L 

PROVIDER: S-EPMC12492650 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature