Efficient <i>trans</i>-aconitic acid production using systematically metabolic engineered <i>Escherichia coli</i>.

ABSTRACT: trans-Aconitic acid (TAA) is a versatile platform biochemical exhibiting extensive applications. In this work, Escherichia coli W3110 (DE3) was metabolic modified for the de novo biosynthesis of TAA. Firstly, a pyruvate accumulation chassis strain E. coli W3110-P6 was constructed through deletion of six byproducts generation genes. Secondly, the aconitate isomerase was screened from four candidates and co-overexpressed with TAA transporter to construct heterologous TAA biosynthetic pathway in E. coli W3110-P6. Thirdly, the genes pyc ^P458S encoding a feedback-insensitive pyruvate carboxylase and gltA encoding an allosterically unaffected citrate synthase were overexpressed, and aceA encoding the isocitrate lyase was deleted, to inc

SUBMITTER: Guo L 

PROVIDER: S-EPMC12492653 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature