Enhanced PrrAB system activation and restricted farnesyl pyrophosphate diversion underlie high coenzyme Q₁₀ accumulation in <i>Rhodobacter sphaeroides</i> HY01.

ABSTRACT: The industrial Rhodobacter sphaeroides HY01 accumulates an exceptionally high level of coenzyme Q₁₀ (Q₁₀), but the underlying mechanisms remain incompletely understood. Given the central role of Q₁₀ in respiratory electron transport, previous observation of reduced expression of cbb ₃ -type cytochrome c oxidase genes in HY01 suggested a potential mechanistic link. In this study, we found that cbb ₃ oxidase activity in HY01 was only 21.8-32.8 % of that in the wild-type 2.4.1, and restoring this activity led to a 64.4 % decrease in Q₁₀ accumulation, demonstrating a strong inverse correlation. This correlation was found to be mediated by the activation of the PrrAB two-component regulat

SUBMITTER: He X 

PROVIDER: S-EPMC12492659 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature