Systems metabolic engineering of dual pathways with stage-specific activation for high-level 5-aminolevulinic acid production in <i>Escherichia coli</i>.

ABSTRACT: 5-Aminolevulinic acid (5-ALA) is an important non-proteinogenic amino acid with wide applications in agriculture and medicine. To achieve high-level microbial production, this study established a dual-pathway reconstruction strategy in Escherichia coli by integrating the endogenous C5 pathway with an inducible exogenous C4 pathway. Multi-copy overexpression of gltX, hemA, and hemL, combined with enhanced glutamate supply and the introduction of a non-oxidative glycolysis pathway, effectively increased the C5 pathway flux and carbon efficiency, while product toxicity was significantly alleviated by strengthening efflux mechanisms and oxidative stress tolerance. A quorum sensing-based regulatory system was employed to dynamically regulate hemB expression, t

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12492688 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature