Evaluating cell viability assessment techniques: a comparative study of flow cytometry and fluorescence microscopy in response to bioactive glass exposure.

ABSTRACT: Reliable in vitro cytotoxicity assessment of biomaterials is essential for preclinical evaluation. Fluorescence microscopy (FM) and flow cytometry (FCM) are widely used methods, yet their comparative performance in particulate systems remains underexplored. This study compares FM and FCM in evaluating the cytotoxicity of Bioglass 45S5 (BG) on SAOS-2 osteoblast-like cells across different particle sizes and concentrations. The cells were treated with BG particles in three size ranges (< 38 µm, 63-125 µm, and 315-500 µm) at concentrations of 25, 50, and 100 mg/mL for 3 and 72 h. FM employs FDA/PI staining to distinguish viable and nonviable cells, whereas FCM utilizes multiparametric staining (Hoechst, DiIC1, Annexin V-FITC, and PI) to classify viable, apoptotic, and necrotic populations. Bo

SUBMITTER: Samuel BJ 

PROVIDER: S-EPMC12492793 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature