Direct identification and antimicrobial susceptibility testing of microorganisms from positive blood culture bottles using a membrane filtration method.

ABSTRACT:

Background

Bloodstream infections (BSIs) are a major cause of morbidity and mortality globally. Rapid and accurate pathogen identification and antimicrobial susceptibility testing (AST) are crucial for timely and effective treatment. Conventional blood culture (BC) workflows are time-consuming, typically requiring 18-48 h. Although integrating matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) with direct AST protocols is promising, certain limitations remain. Therefore, we aimed to establish a faster and more reliable method for the direct identification and AST of microorganisms from positive BC bottles using a membrane filtration-based protocol.

Results

The membrane filtration-based method achieved an overall identification success

SUBMITTER: Liu TP 

PROVIDER: S-EPMC12492815 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature