VISTA expressed on tumor cells is regulated by m6A and influences immune microenvironment through STAT3/CCL22 in NSCLC.

ABSTRACT:

Background

Immunotherapy is playing an increasingly vital role in the treatment of non-small cell lung cancer (NSCLC), yet the challenge of immune evasion remains prevalent. Therefore, investigating the regulatory mechanisms of the tumor immune microenvironment could yield significant benefits.

Methods

This study utilized differential expression analysis, WGCNA, and Lasso-Cox to analyze the GSE126044 dataset, identifying N6-methyladenosine (m6A)-related molecules that play significant roles in immunotherapy. The m6A modification of the molecule were validated through m6A dot blot, MeRIP, RNA pull-down and RNA stability assays. Their impact on the biological behavior of tumor cells (TCs) was evaluated by CCK-8 assays, wound healing assays, transwell assays, co-culture experim

SUBMITTER: Xu H 

PROVIDER: S-EPMC12492831 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature