Urinary microbiome in non-muscle invasive bladder cancer: impact of sample types and sex differences.

ABSTRACT:

Background

Previous research on urinary microbiomes in bladder cancer patients has yielded inconsistent results, highlighting the need for further investigation. This study aims to analyze microbiome dysbiosis in bladder cancer patients by comparing multiple sample types, incorporating negative controls, and assessing sex-based variations. Fifty patients who required transurethral resection of bladder tumor for treatment were selected. Three types of specimens were collected from each patient: midstream urine, catheterized urine, and normal bladder mucosal tissue. Microbiome was analyzed via 16 S rRNA gene amplificon sequencing.

Results

Beta diversity analysis revealed significant differences in microbiome composition between mucosal tissue and urine samples, while no signif

SUBMITTER: Kang C 

PROVIDER: S-EPMC12492864 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature