A yeast-based in vivo assay complements in vitro and in silico approaches to characterize sterol-binding by Saccharomyces cerevisiae Lam/Ltc proteins.

ABSTRACT: Cellular membranes maintain distinct lipid compositions, with sterols enriched in the plasma membrane despite their synthesis in the endoplasmic reticulum (ER). This distribution relies on vesicular and nonvesicular transport, the latter facilitated by lipid transfer proteins (LTPs) at membrane contact sites. In yeast, the Lam/Ltc family of LTPs is critical for sterol transport. To characterize their sterol-binding properties in a cellular context, we developed a yeast-based in vivo assay using Saccharomyces cerevisiae. Here, sterol-binding proteins, fused to signal sequences, extract and export radiolabeled cholesterol from the luminal compartment of the secretory pathway to the culture medium, offering a qualitative measure of binding capacity. We demonstrate that yeast Lam/Ltc proteins

SUBMITTER: El Atab O 

PROVIDER: S-EPMC12493155 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature