A biosynthetic pathway for ornithine lipid formation dependent on a GH3 (Gretchen Hagen 3)-like enzyme in planctomycetes.

ABSTRACT: Amino lipids with acyloxyacyl structures, particularly ornithine lipids (OLs), are widespread in bacteria, but are absent from archaea and eukaryotes. In these lipids, an α-amino acid is N-acylated with a 3-hydroxy fatty acyl residue, and a secondary fatty acid is ester-bound to the hydroxyl group of the first fatty acid. Based on the presence of genes encoding the fatty acid transferases OlsBA or OlsF, involved in ornithine lipid (OL) synthesis, it has been estimated that approximately 50% of sequenced bacterial species can form OL. Here, based on genomic context, structure-based comparisons, and activity essays, we identified a novel pathway for OL synthesis in the planctomycete Singulisphaera acidiphila. This pathway initiates with OlsH, a GH3 (Gretchen Hagen 3)-like enzyme, that cataly

SUBMITTER: Rivera-Najera LY 

PROVIDER: S-EPMC12493201 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature