Performance of disc diffusion and gradient strip test on different Mueller-Hinton agar media plates and ComASP® and Bruker UMIC® Cefiderocol Microdilution Panels for cefiderocol susceptibility testing in carbapenem-resistant Pseudomonas aeruginosa.

ABSTRACT:

Background

Cefiderocol antimicrobial susceptibility testing requires standardized iron levels to produce consistent results. Here we assessed the analytical performances of two commercialized broth microdilution (BMD) methods (Bruker's UMIC and Liofilchem's ComASP), of disc diffusion (DD) and the gradient strip method on a collection of clinical carbapenem-resistant-Pseudomonas aeruginosa (CRPA) isolates.

Methods

A collection of 117 WGS-CRPA was used. Cefiderocol susceptibility was determined (i) by manual BMD (reference method) using iron-depleted Mueller-Hinton (ID-MH), (ii) with the UMIC and ComASP BMD panels, (iii) by DD and (iv) using the gradient strip test, these latter two on bioMérieux MH-agar, Liofilchem MH-agar, and in-house-produced iron-depleted (ID)-MH agar pla

SUBMITTER: Mancini S 

PROVIDER: S-EPMC12494204 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature