Exploring the molecular and biological mechanisms of host response in chickens infected with highly pathogenic avian influenza virus (H5N1): An integrative transcriptomic analysis.

ABSTRACT: The H5N1 strain of highly pathogenic avian influenza (HPAI) has raised worldwide alarm, posing a threat to the health of both animals and humans. The study aimed to identify key genes and biological pathways modulated in chickens infected with H5N1 through meta-analysis. Four microarray datasets from GEO were normalized using RMA, Quantile methods, and the SVA package in RStudio. Differentially expressed genes (DEGs) were screened using the edgR package. Gene enrichment and network analyses were performed, and key genes in the protein-protein interaction (PPI) network were identified using the CytoHubba and MCODE plugins in Cytoscape. Additionally, to investigate the regulatory networks involved, we employed the miRDB web tool to identify microRNAs associated with each hub gene. The result

SUBMITTER: Golpasand S 

PROVIDER: S-EPMC12494259 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature