CENPU promotes tumorigenesis and stem cell properties in triple‑negative breast cancer by suppressing lysosomal furin degradation.

ABSTRACT: Centromere protein U (CENPU), a critical component of the kinetochore complex, structurally integrates with spindle microtubules to mediate chromosome segregation during mitosis. However, the association between CENPU expression levels and tumors is largely unknown. Immunohistochemistry and western blotting were used to analyze CENPU expression and prognostic value in breast cancer tissues. CENPU overexpressing/knockdown cell lines were constructed for 4D‑data‑independent acquisition quantitative proteomics and enrichment analyses. Functional assays, including flow cytometry, mammosphere formation, wound healing and Transwell assay, were used to assess the effects of CENPU on breast cancer stemness, migration and invasion. The associations among CENPU, nerve growth factor (NGF), proNGF and

SUBMITTER: Sun S 

PROVIDER: S-EPMC12494301 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature