Monoclonal Antibodies in the Pathogenesis of Heparin-Induced Thrombocytopenia.

ABSTRACT:

Background

Heparin-induced thrombocytopenia (HIT) is an immune-mediated platelet disorder caused by antibodies that target complexes of platelet factor 4 (PF4) and heparin. HIT has been characterized as a polyclonal immune response; however, studies of other rare anti-PF4 disorders have identified clonally restricted antibodies.

Methods

In this study, we investigated the clonality of pathogenic HIT antibodies. Antibodies against PF4-heparin were affinity-purified with the use of PF4-heparin beads from serum samples obtained from nine patients with clinically and serologically confirmed HIT. Antibody clonality was assessed by means of immunofixation electrophoresis and mass spectrometry. Antibody binding to PF4 was evaluated by an enzyme immunoassay, and functional platelet a

SUBMITTER: Treverton J 

PROVIDER: S-EPMC12494375 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature