Mapping cellular cytosolic pH in vivo under physiological and pathophysiological conditions.

ABSTRACT: Cytosolic pH (pHc) is one of the most essential intracellular microenvironments profoundly affecting charges, conformations and therefore functions of macromolecules. It remains challenging to quantitatively measure pHc at single-cell resolution in vivo, and how pHc of a specific cell type dynamically responds to physiological or pathophysiological fluctuations remains elusive. Smad5 is a pHc sensor with accelerated nuclear export upon pHc alkalization and ideally the nucleocytoplasmic ratio of Smad5 would be a potential indicator of pHc. Here, we revealed that the constitutive eGFP-Smad5 expression transgenic mice could serve as pHc reporter for quantitative detection of cellular pHc in vivo. We mapped pHc of major cell types in the reporter mice and found robust pHc heterogeneity in indi

SUBMITTER: Zhang S 

PROVIDER: S-EPMC12494554 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature