ScRNA-seq deciphers molecular mechanisms of endocrine disruptor 4-nonylphenol impairing spermatogenesis in mice.

ABSTRACT: 4-Nonylphenol (NP) is an environmental endocrine disruptor widely used in consumer products. Previous studies have shown that NP can interfere with hormone synthesis and metabolism in humans and animals, leading to male reproductive dysfunction. This study utilized the scRNA-seq method to evaluate cell populations and their heterogeneity, aiming to elucidate the toxic mechanisms of NP exposure on testicular cells. We demonstrate, for the first time, the transcriptomic characteristics of testicular single cells in adolescent mice exposed to NP. Adolescent mice, initially exposed at 4 weeks of age, were subsequently analyzed at sexual maturity after a continuous exposure period of 3 months. The blank control and NP-exposed groups underwent scRNA-seq analysis, identifying ten cell populations

SUBMITTER: Zhao X 

PROVIDER: S-EPMC12494639 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature