ID8 cells manifest phenotypic plasticity and molecular heterogeneity of high-grade serous ovarian cancer in response to the local tissue niche.

ABSTRACT: Spontaneously transformed mouse ovarian surface epithelial cell line ID8 generates a syngeneic mouse model, recapitulating the primary and metastatic stages of the malignancy hence, frequently used in pre-clinical evaluation. Due to inherent phenotypic plasticity, primary and metastatic tumors show contrasting phenotypic features dictated by the tissue niche. To address the tumor heterogeneity within the model we stratified it into a specific molecular subtype, which would provide an accurate setting for interpretation of experimental data. Stratification was performed on ID8 cell line and ID8 generated xenografts based on immunostaining, live cell imaging, histopathology, transcriptomics and proteomics. Tumor microenvironment (TME) investigation for each xenograft was done through flow cy

SUBMITTER: Gupta R 

PROVIDER: S-EPMC12494684 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature