Variant scoring tools for deep mutational scanning.

ABSTRACT: Deep mutational scanning (DMS) can systematically assess the effects of thousands of genetic variants in a single assay, providing insights into protein function, evolution, host-pathogen interactions, and clinical impacts. Accurate scoring of variant effects is crucial, yet the diversity of tools and experimental designs contributes considerable heterogeneity that complicates data analysis. Here, we review and compare 12 computational tools for processing DMS sequencing data and scoring variant effects. We systematically outline each tool's statistical approaches, supported experimental designs, input/output requirements, software implementation, visualisation capabilities, and key assumptions. By highlighting the strengths and limitations of these tools, we hope to guide researchers in s

SUBMITTER: Cubuk H 

PROVIDER: S-EPMC12494760 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature