Patch2MAP combines patch-clamp electrophysiology with super-resolution structural and protein imaging in identified single neurons without genetic modification.

ABSTRACT: Recent developments in super-resolution microscopy have revolutionized the study of cell biology. However, dense tissues require exogenous protein expression for single cell morphological contrast. In the nervous system, many cell types and/or species of interest - particularly humans - are not amenable to genetic modification and exhibit intricate anatomical specializations which make cellular and subcellular delineation challenging. Here, we present a method we call Patch2MAP for full morphological labeling of individual neurons from any species or cell type for subsequent cell-resolved protein analysis at the nanoscale. By combining patch-clamp electrophysiology with epitope-preserving magnified analysis of proteome (eMAP), our method additionally allows for correlation of physiological

SUBMITTER: Vardalaki D 

PROVIDER: S-EPMC12494780 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature