Ontology highlight
ABSTRACT:
SUBMITTER: Vincent M
PROVIDER: S-EPMC1249482 | biostudies-literature | 2004 Aug
REPOSITORIES: biostudies-literature
Vincent Myriam M Xu Yan Y Kong Huimin H
EMBO reports 20040709 8
Polymerase chain reaction is the most widely used method for in vitro DNA amplification. However, it requires thermocycling to separate two DNA strands. In vivo, DNA is replicated by DNA polymerases with various accessory proteins, including a DNA helicase that acts to separate duplex DNA. We have devised a new in vitro isothermal DNA amplification method by mimicking this in vivo mechanism. Helicase-dependent amplification (HDA) utilizes a DNA helicase to generate single-stranded templates for ...[more]