Single Cell Profiling in the Sox10^Dom Hirschsprung Mouse Implicates Hox Genes in Enteric Neuron Trajectory Allocation.

ABSTRACT:

Background & aims

Enteric nervous system (ENS) development requires migration, proliferation, and differentiation of progenitors for normal gastrointestinal (GI) motility. Sox10 deficit causes aganglionosis, modeling Hirschsprung disease (HSCR), and disrupts ratios of postnatal enteric neurons in proximal ganglionated bowel. How Sox10 deficiency alters enteric neuron ratios is unclear. Sox10's prominent expression in enteric neural crest-derived progenitors (ENCPs) and lack of this gene in mature enteric neurons led us to examine Sox10^Dom effects in early ENS development.

Methods

Immunohistochemistry localized SOX10 in the developing ENS relative to HuC/D. ENS progenitors, developing neurons, and enteric glia were isolated from Sox10^+/+ and Sox10^Do

SUBMITTER: Avila JA 

PROVIDER: S-EPMC12494863 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature