Quantitative essentiality in a reduced genome: a functional, regulatory and structural fitness map.

ABSTRACT: Essentiality studies have traditionally focused on coding regions, often overlooking other small genetic regulatory elements. To address this, we combined transposon libraries containing promoter or terminator sequences to obtain a high-resolution essentiality map of a genome-reduced bacterium, at near-single-nucleotide precision when considering non-essential genes. By integrating temporal transposon-sequencing data by k-means unsupervised clustering, we present a novel essentiality assessment approach, providing dynamic and quantitative information on the fitness contribution of different genomic regions. We compared the insertion tolerance and persistence of the two engineered libraries, assessing the local impact of transcription and termination on cell fitness. Essentiality assessment

SUBMITTER: Miravet-Verde S 

PROVIDER: S-EPMC12494982 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature