A dual fluorescent-Raman bioorthogonal probe for specific biosynthetic labeling of intracellular gangliosides.

ABSTRACT: Gangliosides are sialic acid-containing glycosphingolipids integral to the cell membrane, and they are particularly abundant in the nervous system. Aberrant ganglioside metabolism contributes to pathological conditions, including neurodegenerative diseases, lysosomal storage disorders, and cancer. A critical precursor for sialic acid biosynthesis is N-acetyl-D-mannosamine (ManNAc), which can be epimerized from the corresponding UDP-GlcNAc or exogenously supplied through ManNAc derivatives. Currently, tools to visualize and detect gangliosides are very limited and non-specific. Here, we describe a dual fluorescent and Raman-active ManNAlk derivative, phenanthrene-9-Pr₄ManNAlk (MM-JH-2), capable of one-step selective labeling of gangliosides in cells. This modified ManNAlk derivat

SUBMITTER: Mukherjee MM 

PROVIDER: S-EPMC12494992 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature