From Low-Loaded Mesophilic to High-Loaded Thermophilic Anaerobic Digestion: Changes in Reactor Performance and Microbiome.

ABSTRACT: This study investigated temporal dynamics in reactor performance and microbial community structure during anaerobic digestion of sewage sludge when the temperature was changed from 37°C to 55°C, followed by an increase in organic loading rate (OLR). Performance instability was observed immediately following the temperature increase and in the end of the study when the OLR was 11.1 ± 0.3 kgVS m^-3d^-1. The specific methane production peaked at 0.31 ± 0.06 Nm³ kg^-1 volatile solids (VS) during thermophilic operation and when the OLR was 3.5 ± 0.9 kgVS m^-3d^-1. Using metagenomic sequencing, 304 species-representative genome bins (SGB) were assembled. Network analysis revealed that 186 SGB were associated with thermophilic condition

SUBMITTER: Modin O 

PROVIDER: S-EPMC12495138 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature