H₂S-Mediated GH3.1 Persulfidation Regulates IAA Homeostasis to Enhance Nodulation Formation and Nitrogen Fixation in Robinia pseudoacacia.

ABSTRACT: Hydrogen sulphide (H₂S), a gaseous signalling molecule, plays a multifaceted role in plant physiology by enhancing adaptability to environmental stresses. However, the regulatory mechanism of symbiotic nitrogen (N) fixation by H₂S in indeterminate nodules of woody legumes remains unclear. In this study, we investigated the mechanism by which H₂S promotes nodulation and N fixation in the woody legume Robinia pseudoacacia. Exogenous H₂S significantly enhanced rhizobium infection, nodule formation and nitrogenase activity, demonstrating its positive role in the symbiotic process. Transcriptomic analysis of roots and nodules revealed that H₂S signalling modulates auxin metabolism, particularly through the regulation of indole-3-acetic acid

SUBMITTER: Zhang W 

PROVIDER: S-EPMC12495139 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature