Postnatal alterations in skeletal muscle fiber composition of myostatin-knockout chickens.

ABSTRACT: In a previous study, we identified a hypermuscular phenotype attributed to both muscle fiber hyperplasia and hypertrophy in myostatin (MSTN)-knockout (KO) chickens generated by clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR-Cas9) technology. In mammals, MSTN deletion results in increased production of fast glycolytic fibers, accompanied by decreased production of slow oxidative fibers. However, the effects of MSTN deletion on muscle fiber types have rarely been studied in avian species such as chickens. In this study, we analyzed muscle fibers in chickens at various ages to investigate whether MSTN deletion alters muscle fiber composition after hatching. Immunofluorescence and histochemical staining, including ATPase and nicotinamide adenine dinucleo

SUBMITTER: Kim SE 

PROVIDER: S-EPMC12495153 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature