In-situ synthesized porphyrinic zirconium MOF-derived multilayered nanocomposite probe for ultra-sensitive immunosensor.

ABSTRACT: Aflatoxin B₁ (AFB₁) is a major concern due to its widespread contamination and potential harm. Colorimetric lateral flow immunoassays (LFIAs) incorporating gold nanoparticles (AuNPs) are widely used for on-site analyte detection. However, it remains challenging due to the limited brightness of AuNPs. Herein, we introduce an immunosensor for highly sensitive AFB₁ detection utilizing a multilayered nanocomposite probe (PCN-224@Fe₃O₄@PDA@Ab/HRP), created by sequentially modifying Fe₃O₄ nanoparticles, polydopamine layers, and horseradish peroxidase on a porphyrinic metal-organic framework, which offers magnetic separation, high signal brightness, and strong catalytic activity. Integrated with a competitive assay forma

SUBMITTER: Li X 

PROVIDER: S-EPMC12495349 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature