Accurate and Cost-Efficient Whole Genome Sequencing of Hepatitis B Virus Using Nanopore.

ABSTRACT: Deep sequencing of the whole hepatitis B virus genome increases the analytical resolution and has the potential to improve molecular epidemiology investigations. The aim of this study was to develop and evaluate the performance of such deep sequencing using the Nanopore technology. The method includes an initial PCR step to generate two overlapping amplicons that cover the whole relaxed circular HBV genome found in circulating viral particles and covalently closed circular DNA in infected hepatocytes, followed by sequencing using the Nanopore rapid barcoding kit that allows parallel analysis of several samples in one reaction. The libraries can be sequenced with the standard Nanopore flow cell on MiniIon or GridIon devices, as well as the Flongle. The performance of the method was evaluate

SUBMITTER: Stenback JB 

PROVIDER: S-EPMC12495540 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature