Tetrahydropalmatine has analgesic role in mouse model of bone cancer pain by inactivating the TNF-α/uPA/PAR2/TRPV1 pathway in dorsal root ganglia.

ABSTRACT:

Background

Treatment of bone cancer pain (BCP) remains a challenge. The current paper was to research the analgesic effect of Tetrahydropalmatine (THP) on BCP and the related mechanisms.

Methods

Mouse model of BCP was constructed by injecting E0771 breast cancer cells into the tibia. Behavioral test was performed to research the effect of THP on pain nociception of BCP mice. Tibia and dorsal root ganglia (DRG) damage was evaluated by HE and Nissl staining. The construction of BCP cell models was conducted by co-culture DRG neurons with E0771 breast cancer cells. The effect of THP on the viability and apoptosis of BCP cell models was monitored by CCK-8 and Tunel assays. TNF-α/uPA/PAR2/TRPV1 pathway activity in DRG was detected by qRT-PCR and Western blot. Pomalidomide (PMA),

SUBMITTER: Zheng G 

PROVIDER: S-EPMC12495623 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature