Computational evidence for hundreds of non-conserved plant microRNAs.

ABSTRACT:

Background

MicroRNAs (miRNA) are small (20-25 nt) non-coding RNA molecules that regulate gene expression through interaction with mRNA in plants and metazoans. A few hundred miRNAs are known or predicted, and most of those are evolutionarily conserved. In general plant miRNA are different from their animal counterpart: most plant miRNAs show near perfect complementarity to their targets. Exploiting this complementarity we have developed a method for identification plant miRNAs that does not rely on phylogenetic conservation.

Results

Using the presumed targets for the known miRNA as positive controls, we list and filter all segments of the genome of length approximately 20 that are complementary to a target mRNA-transcript. From the positive control we recover 41 (of 92 possi

SUBMITTER: Lindow M 

PROVIDER: S-EPMC1249568 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature