Effect of tumor draining lymph nodes in the formation and maturation of tertiary lymphoid structure in patients with lung adenocarcinoma.

ABSTRACT:

Background

Tertiary lymphoid structures (TLSs), ectopic lymphoid aggregates composed of immune cells, are associated with favorable clinical outcomes and increased efficacy of anti-tumor immunotherapies. However, the mechanisms underlying the formation and maturation of TLSs require further elucidation. The correlation between tumor immune microenvironment in tumor-draining lymph nodes (TDLNs) and TLSs remains inadequately studied. The study aimed to utilize multiplex immunofluorescence (mIF) to explore the relationship between TDLN and TLSs.

Methods

Tissue slides from 120 patients with lung adenocarcinoma (LUAD) were collected to perform for mIF staining. Panel 1 (DAPI, CD20, CD21, CD23) was used for the quantitative and qualitative analyses of TLS. Panel 2 (DAPI, CD4, CD8,

SUBMITTER: Wen J 

PROVIDER: S-EPMC12495740 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature