α-hederin inhibits cervical cancer progression by inducing DNA damage-dependent cell cycle blockade and apoptosis.

ABSTRACT:

Background

Cervical cancer incidence has not decreased significantly despite widespread use of HPV vaccines and screening measures.

Purpose

This study explored the potential of α-Hederin in the treatment of cervical cancer.

Methods

CCK8, EdU staining assay, flow cytometry were conducted for apoptosis, cell cycle, ROS, and mitochondrial membrane potential. Mechanism effects of α-hederin on cervical cancer cells were investigated using transcriptome sequencing, bioinformatics analysis, and single-cell sequencing. Further in-depth detection of key proteins with western blot, immunofluorescence, overexpression of CHK1 gene, DNA damage inhibitors, and NAC inhibitors were performed to study the regulation of the cell cycle. In vivo nude mice tumorigenicity experiments and m

SUBMITTER: Song Y 

PROVIDER: S-EPMC12495757 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature