Exploratory high-throughput screening of repurposed drugs for canine lymphoid malignancies.

ABSTRACT:

Background

Lymphoid malignancies are common in dogs. However, the limitations of existing chemotherapy highlight the need for alternative therapies. Drug repositioning is a promising approach for discovering new therapies using existing drugs. In this study, we conducted high-throughput screening (HTS) of clinically used drugs to identify candidates with antiproliferative activity against canine lymphoid tumor cells in vitro.

Methods

A total of 1,824 compounds were screened at 5 µM through HTS using a water-soluble tetrazolium assay in three canine lymphoid tumor cell lines (GL-1, UL-1, CLBL-1) and one non-tumorigenic epithelial cell line (MDCK). Compounds that selectively inhibited tumor cells while sparing the MDCK cells were retained as primary screening candidates. Compo

SUBMITTER: Nishida M 

PROVIDER: S-EPMC12495782 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature