Environmental Dissemination of Multidrug-Resistant Gram-Negative Pathogens in Djibouti's Wastewaters.

ABSTRACT:

Background

The occurrence of multidrug-resistant (MDR) Enterobacteriaceae-producing extended-spectrum β-lactamases (ESBLs) and/or carbapenemases is increasingly reported in clinical and environmental settings. Here, we aimed to investigate the resistance profile of gram-negative bacterial isolates from urban and environmental wastewater in Djibouti.

Materials and methods

Twenty-five wastewater samples were collected from eight different locations of Djibouti. After bacterial culture on selective media for gram-negative bacteria (GNB), obtained isolates were identified using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Antibiotic susceptibility testing was performed by disk diffusion and E-test methods. Standard PCR and Sang

SUBMITTER: Ragueh AA 

PROVIDER: S-EPMC12495887 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature