Mode-Specific Coherent Interference of Vibrational Sum-Frequency Generation Imaging: An Approach to Differentiate Lung Tumors through Collagen Interfibrillar Distances.

ABSTRACT: Chemical imaging holds great promise for chemical, materials, and biological applications. However, its contrast often relies on subtle spectral differences arising from molecular-level changes. Here, we introduce label-free chemical imaging based on bond-specific coherent interference, which is highly sensitive to nanoscopic structural variations in collagen fibers. We demonstrated this idea using vibrational sum-frequency generation (VSFG) microscopy to identify lung tumors by detecting collagen I structural remodeling. Hyperspectral VSFG images reveal dramatic differences in the NH and CH_x stretch regions between metastatic and tumor-free lung tissues. Based on these differences, key spectral signatures, such as the intensity ratio of NH_S/CH_2,Ss a

SUBMITTER: Yang B 

PROVIDER: S-EPMC12496127 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature