Characterization of human CD34⁺ HSPC-derived neutrophils with limited myeloid-derived immunosuppressive cell activity.

ABSTRACT: Neutrophils have limited utility as a fundamental research model and transfusion product due to their short lifespan. Here, we cultured CD34⁺ hematopoietic stem and progenitor cell (HSPC)-derived neutrophils and compared them to peripheral blood neutrophils in terms of morphology, phenotype, and function. Our culture system resulted in morphologically mature CD15⁺CD11b⁺CD16^high neutrophils with effector functions almost indistinguishable from blood neutrophils, confirmed by a high similarity in transcription and protein abundance patterns. While exhibiting microbial killing capacity and antibody-dependent cellular cytotoxicity, these cells were deficient in myeloid-derived suppressor cell activity. This deficiency in immunosuppressive activity co

SUBMITTER: Webbers SDS 

PROVIDER: S-EPMC12496184 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature