Novel monoclonal antibodies for immunodetection of AmpC β-lactamases.

ABSTRACT:

Background

Accurate and easy-to-perform assays for the detection of antibiotic-resistant bacterial isolates producing AmpC β-lactamases are epidemiologically relevant, leading to more effective use of antibiotics and a comprehensive understanding of β-lactamase prevalence. We describe novel monoclonal antibodies (MAbs) against CMY β-lactamases and their application in immunoassays for the detection of CMY-producing bacterial isolates.

Methods

Recombinant CMY-34 was expressed in Escherichia coli and used as an immunogen for MAb generation by hybridoma technology. Selected CMY-34-specific MAbs were comprehensively characterized by various immunoassays, computational analysis, and sequencing of their variable domains. To prove MAb reactivity with CMY β-lactamases, the an

SUBMITTER: Bielske K 

PROVIDER: S-EPMC12497404 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature