A broad-spectrum lasso peptide antibiotic targeting the bacterial ribosome.

ABSTRACT: Lasso peptides (biologically active molecules with a distinct structurally constrained knotted fold) are natural products that belong to the class of ribosomally synthesized and post-translationally modified peptides^1-3. Lasso peptides act on several bacterial targets^4,5, but none have been reported to inhibit the ribosome, one of the main targets of antibiotics in the bacterial cell^6,7. Here we report the identification and characterization of the lasso peptide antibiotic lariocidin and its internally cyclized derivative lariocidin B, produced by Paenibacillus sp. M2, which has broad-spectrum activity against a range of bacterial pathogens. We show that lariocidins inhibit bacterial growth by binding to the ribosome and interfering with protein synthesis.

SUBMITTER: Jangra M 

PROVIDER: S-EPMC12497486 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature