Targeting Ferroptosis With Ginsenoside Rg3 Alleviates Intervertebral Disc Degeneration.

ABSTRACT:

Objective

Intervertebral disc degeneration (IVDD) has been closely associated with ferroptosis in nucleus pulposus cells (NPCs), the underlying regulatory mechanisms and therapeutic strategies remain poorly defined. This study aims to delineate how ginsenoside Rg3 mitigates IVDD progression through ferroptosis suppression, providing a basis for clinical translation.

Method

An erastin-induced nucleus pulposus cell ferroptosis model was established. Suitable Erastin concentrations (0-20 μM) were screened via CCK-8, qRT-PCR, and Western blotting based on viability, extracellular matrix (COL2A1/ACAN/ADAMTS5/MMP3) and ferroptosis markers (GPX4/FTH-1/ACSL4), followed by determination of optimal Rg3 concentrations (0-150 μM) using identical methods. Key targets of Rg3 were predicte

SUBMITTER: Xu W 

PROVIDER: S-EPMC12497545 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature