High-throughput synaptic connectivity mapping using in vivo two-photon holographic optogenetics and compressive sensing.

ABSTRACT: Characterizing synaptic connectivity in living neural circuits is key to understanding the interplay between network structure and function during behavior. However, the throughput of current in vivo synaptic mapping methods remains very limited. Here, we present a framework for increasing mapping throughput and speed that combines two-photon holographic optogenetic stimulation of presynaptic neurons, whole-cell recordings of postsynaptic responses and compressive sensing reconstruction of sparse connectivity. Under sequential single-cell stimulation, the method enables rapid probing of connectivity across up to 100 potential presynaptic cells within ~5 min in the visual cortex of anesthetized mice, identifying synaptic pairs along with their strength and spatial distribution. Furthermore,

SUBMITTER: Chen IW 

PROVIDER: S-EPMC12497651 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature