USP8, USP48, BRAF and TP53 mutations in crooke cell adenoma.

ABSTRACT:

Purpose

Crooke cell adenomas (CCAs) are rare histological subtype of corticotroph pituitary adenomas (cPAs) commonly related to worse prognosis in patients. Notable progress in understanding of the molecular background of cPAs has been made recently but biology of CCAs remains poorly recognized. Results of our previous study suggested distinct frequency of the known recurrent mutations in CCAs than in sparsely and densely granulated cPAs. Thus, the aim was to determine the prevalence of USP8, USP48, BRAF and TP53 variants in a relatively large retrospective group of patients diagnosed with CCA.

Methods

DNA was isolated from formalin-fixed and paraffin-embedded tissue of 29 CCAs (14 clinically functioning and 15 nonfunctioning). Sanger sequencing was used for the identificati

SUBMITTER: Kober P 

PROVIDER: S-EPMC12497664 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature