Single-cell RNA sequencing reveals cell immune status and dysregulated monocytes in patients with myasthenia gravis.

ABSTRACT:

Objectives

As an autoimmune disorder, myasthenia gravis (MG) manifests as an autoimmune attack on postsynaptic neuromuscular junction proteins by pathogenic autoantibodies. This immune attack disrupts neurotransmission, resulting in fatigable skeletal muscle weakness with diurnal fluctuation. However, functional cure biomarkers for patients remain limited.

Methods

Peripheral blood collection was performed at three time points in patients with MG: before treatment (Pre), 1 month after treatment (Post) and functional cure (long-term follow-up, LF). Single-cell RNA sequencing was performed. The clinical examination results were collected and summarised.

Results

In general, patients with MG exhibited dynamic changes in immune cell composition and inflammatory features. In

SUBMITTER: Guo Y 

PROVIDER: S-EPMC12497684 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature